This state-wide Conference was hosted by the Colorado Department of Public Health and Environment, Colorado State University (SCU) One Health Institute, and the Rockies and High Plains Vector-borne Disease Training and Evaluation Center. It covered One Health and zoonotic disease topics such as the impact of climate change on vector-borne diseases, the current Highly Pathogenic Avian Influenza outbreak that is impacting many wild and domestic animals, including wild birds and mammals, poultry, dairy cattle and people; WNV surveillance; One Health surveillance for bat-associated viruses, and more. Over 300 participants and thirteen speakers.